Determination of antifungal activity and presence of a lipid transfer peptide in Chamaemelum nobile

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Published: Feb 28, 2025
DOI: https://doi.org/10.17163/lgr.n41.2025.07
Keywords:
Roman chamomile, Antimicrobial peptide, Lipid transfer protein, Rhizoctonia solani

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Diana Daniela Portela Dussán
Faculty of Sciences, Universidad Antonio Nariño, Carrera 3 Este 47A-15, Bogotá D.C., Colombia.
https://orcid.org/0000-0003-2949-7033
Sandra Mónica Estupiñan Torres
Faculty of Health Sciences, Universidad Colegio Mayor de Cundinamarca, Calle 28 No. 5B-02 Bogotá D.C., Colombia.
https://orcid.org/0000-0002-6937-4567
Silvio Alejandro Lopez-Pazos
Faculty of Sciences, Universidad Antonio Nariño, Carrera 3 Este 47A-15, Bogotá D.C., Colombia.
https://orcid.org/0000-0002-3682-4162

Abstract

Chamaemelum nobile, or Roman chamomile, is a plant containing anti-inflammatory and antimicrobial properties. Antimicrobial peptides (AMPs) are part of the plant defense system including lipid transfer peptides (LPTs). Our objective is to identify a LTP-related protein from C. nobile (cnLTP). PCR was performed on C. nobile DNA for identifying cnLTP gene. Bioinformatics was used for their characterization, and a sensitivity test was carried out on Rhizoctonia solani. cnLTP has 99 amino acids, 9.8 kDa, isoelectric point of 9.39, 33 aliphatic residues, aliphatic index of 85, hydropathicity of 0.127, four alpha-helices and four disulfide bridges. An inhibitory activity of apoplastic fluid of C. nobile was determined at 1 µg/mL on R. solani. This study contributes in the knowledge of a novel and non-characterized LTP using in silico and experimental related approaches.

Article Details

Issue
Vol. 41 No. 1 (2025): (March 2025-August 2025)
Section
Scientific Article
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