Evaluation of PLA active biodegradable films incorporated of essential oils to inhibit microbial adhesion

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Published: Jul 27, 2022
Updated: 2022-07-27
Versions:
2022-07-27 (2)
DOI: https://doi.org/10.17163/lgr.n36.2022.02
Keywords:
biopolymer, food, barrier, infectious agent

Main Article Content

Anderson Pazmiño
Universidad Técnica de Manabí
https://orcid.org/0000-0001-5333-3819
Ana Campuzano
Universidad Agraria del Ecuador
https://orcid.org/0000-0003-0010-4267
Karina Marín
Escuela Superior Polítécnica del Litoral
https://orcid.org/0000-0003-0010-4267
Jonathan Coronel
Escuela Superior Politécnica del Litoral
https://orcid.org/0000-0001-6535-0261
Rómulo Salazar
Escuela Superior Politécnica del Litoral
https://orcid.org/0000-0002-0172-839X

Abstract

An evaluation of incorporated polylactic acid (PLA) active biodegradable films of essential oils to inhibit microbial adhesion was performed using a fractional exploratory design, combining the factors, amount of PEG 400 plasticizer (10 and 20%), amount of cinnamon essential oil (0.5% and 1%), amount of oregano essential oil (0.5% and 1%), Salmonella spp. concentration (103 CFU mL-1 and 104 CFU mL-1) and Staphylococcus aureus concentration (103 CFU mL-1 and 104 CFU mL-1). The results of microbial adhesion inhibition test showed that the maximum inhibition percentage reached 73.82 ± 0.35 % corresponding to experiment 7 (bce), which contains 10% of PEG 400, 1% of cinnamon essential oil, 1% of oregano essential oil, 103 CFU mL-1 of Salmonella spp. concentration, 104 CFU mL-1 of Staphylococcus aureus concentration. Statistical analysis determined that there is strong significant evidence (p-value = 0.0283) that Staphylococcus aureus concentration influences the inhibition percentage to microbial adhesion; as well as that the cinnamon essential oil- Salmonella spp. interaction has little significant evidence (p-value = 0.0711) that influences the inhibition percentage. Inhibition results greater than 60% have the highest concentration of Staphylococcus aureus as a common factor. PLA active biodegradable films with a higher inhibition percentage can potentially be used in the food industry as a barrier mechanism to avoid bacterial contamination.

Article Details

Issue
Vol. 36 No. 2: (September 2022-February 2023).
Section
Scientific Article
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