Identificación de un péptido antimicrobiano de Chamaemelum

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Publicado: Feb 28, 2025
DOI: https://doi.org/10.17163/lgr.n41.2025.07
Palabras clave:
Manzanilla romana, péptido antimicrobiano, proteína de transferencia de lípidos, Rhizoctonia solani

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Diana Daniela Portela Dussán
Faculty of Sciences, Universidad Antonio Nariño, Carrera 3 Este 47A-15, Bogotá D.C., Colombia.
https://orcid.org/0000-0003-2949-7033
Sandra Mónica Estupiñan Torres
Faculty of Health Sciences, Universidad Colegio Mayor de Cundinamarca, Calle 28 No. 5B-02 Bogotá D.C., Colombia.
https://orcid.org/0000-0002-6937-4567
Silvio Alejandro Lopez-Pazos
Faculty of Sciences, Universidad Antonio Nariño, Carrera 3 Este 47A-15, Bogotá D.C., Colombia.
https://orcid.org/0000-0002-3682-4162

Resumen

Chamaemelum nobile, o manzanilla romana, es una planta que contiene propiedades antiinflamatorias y antimicrobianas. Los péptidos antimicrobianos (AMP) son parte del sistema de defensa de las plantas, que incluye a los péptidos de transferencia de lípidos (LPT). Nuestro objetivo es identificar una proteína relacionada con LTP de C. nobile (cnLTP). Se realizó la PCR sobre el ADN de C. nobile para la identificación del gen cnLTP; se utilizó bioinformática para su caracterización, y una prueba de sensibilidad contra Rhizoctonia solani. cnLTP tiene 99 aminoácidos, 9,8 kDa, punto isoeléctrico de 9,39, 33 residuos alifáticos, índice alifático de 85, hidropaticidad de 0,127, cuatro hélices alfa y cuatro puentes disulfuro. Se encontró que el fluido apoplásico de C. nobile (1 µg/mL) inhibe el desarrollo de R. solani. Este estudio contribuye al conocimiento de un LTP novedoso y no caracterizado, utilizando enfoques in silico y experimentales.

Detalles del artículo

Número
Vol. 41 Núm. 1 (2025): (marzo 2025 -agosto 2025)
Sección
Artículo Científico
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