Determination of the reference values in the blood count of horses born or reared more than 3000 m.a.s.l. in the northcentral highlands of Ecuador

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José Luis Izurieta Barzola
Diego Luna
Yolanda Mercedes Cedeño Prócel
Sergio Rolando Chacha Vega

Keywords

Erythropoietin, hemoglobin, hemogram, horses, hypoxia.

Abstract

In the north central Ecuadorian highland, a blood count was performed on 100 horses over two years of age, raised over 3000 m.a.s.l., the objective of this investigation was to determine hematological values at this altitude and compare them with other studies performed at sea level. The blood samples were taken at rest from the animals and sent to the laboratory to be analyzed in the automatic hematology equipment Mindray R BC2800Vet, for the analysis of the data and to determine the reference values we used the Microsoft Excel add-on R Reference Value Advisor. Samples were taken from Ecuadorian Creole horses to obtain hematological data of this type of horses previously untested. Was found 82 samples suitable for establishing blood count values with normal distribution, red blood cells 6,23−10,84×106/mL; hemoglobin 11,4−18,4 g/L; hematocrit 32,3−52,3%, mean corpuscular volume 40,2−57 fL; Mean corpuscular hemoglobin 14−20,3 pg; Mean corpuscular hemoglobin concentration 32−37 g/L; white blood cells 4,8−12×103/mL; lymphocytes 1,4−7,5×103/mL; monocytes 0,2−0,7×103/mL; granulocytes 2,2−8,8×103/mL; lymphocytes% 16,8 − 64,9%, monocytes% 2,9 − 7%, granulocytes% 30,5 − 78,7%; platelets 101 − 401 × 103/mL. The results were compared with international bibliographical references and significant differences (P< 0,05) in red blood cells, hemoglobin, hematocrit and monocytes were found in the study of Böhmwald et al., (1984), a significant difference in hematocrit, mean corpuscular volume, lymphocytes, monocytes and platelets with the study of Giordano et al., (2008); While with the study of Díaz et al., (2011) significant difference with hemoglobin, monocytes and platelets. 
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