Microbiological Evaluation And Chemical Composition Of Organic Extracts From Euphorbia aff. viridis (Klotzsch & Garcke) Boiss ON Staphylococcus Aureus, Klebsiella Pneumoniae AND Escherichia Coli

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Mónica Judith Espadero Bermeo https://orcid.org/0000-0002-2608-9387
Hernán Avilés
Lilia Marlene Armijos Rodríguez https://orcid.org/0000-0003-0802-3701
Luis Enrique Ávila Granda https://orcid.org/0000-0001-6576-2696
Lidia Idrovo https://orcid.org/0000-0001-9875-9477
María Idrovo https://orcid.org/0000-0001-9711-0916
Christian Oyola https://orcid.org/0000-0003-2289-5688

Keywords

Antimicrobial, organic extract, GC-MS, Euphorbia aff. viridis (Klotzsch Garcke) Boiss.

Abstract

Euphorbia aff. Viridis (Klotzsch & Garcke) boiss, a plant species belonging to the Euphorbiaceae family, is one of the most diverse of the American continent, and is used in multiple treatments in ancestral medicine in the Ecuadorian Andes. In the present study organic compounds extracted from the leaves of the plant were analyzed. The description and taxonomic classification of the plant was established. For the phytochemical study, phenols, tannins, saponins, coumarins, lactones and flavonoids were analyzed, and positive results were observed for each chemical compound, with the exception of alkaloid compounds. The chemical characterization by Gas Chromatography coupled to mass spectrometry GS-MS, showed 36.66% lanosterol, 12.25% β-sitosterol, 5.11% 3-β-cholest-4-en-3-ol, 4.73% Hexadecanoic acid as major elements. The microbiological assay of ethanolic extracts showed a 44% inhibition percentage on Klebsiella pneumoniae (ATCC70693) and 43% on Escherichia coli (ATCC10536) with an MIC of 30μl / ml for each strain, and a negative inhibition result was observed for Staphylcoccus aureus (ATCC24213). The organic extracts of Euphorbia aff. Viridis (Klotzsch & Garcke) Boiss, presented antimicrobial activity being able to observe their potential use as antimicrobial agents.
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